Investigations of the bioprocess parameters for the production of hemicellulases by Thermomyces lanuginosus strains
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Abstract
The aim of this study was to evaluate T. lanuginosus for the production of hemicellulases, its yield enhancement using mutagenesis and application of a selected xylanase on bagasse pupl to assess the improvement of pulp properties.
The objectives were:
To determine the localization of hemicellulases in T. lanuginosus strains,
To develop high yielding strains of T. lanuginosus through mutagenensis,
To investigate the synthesis of xylanase by T. lanuginosus MC134,
To optimize the medium components and cultural conitions of T. lanuginosus MC134 strain,
To study the influence of agitation and aeration on the production of xylanase by T. lanuginosus MC134 in a fermenter,
To evaluate the bleach boosting abilities of T. lanuginosus xylanase on bagasse pulp,
To evaluate simultaneous xylanase production and biobleaching potential of T. lanuginosus.
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Submitted in fulfilment for the requirement of a Degree of Doctor of Technology: Biotechnology, Durban University of Technology, Durban, South Africa, 2010.
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https://doi.org/10.51415/10321/740