Please use this identifier to cite or link to this item: https://hdl.handle.net/10321/5747
Title: Biochemical and in silico structural properties of a thermo-acid stable β-glucosidase from Beauveria bassiana
Authors: Magwaza, Buka 
Amobonye, Ayodeji
Bhagwat, Prashant 
Pillai, Santhosh
Keywords: Beauveria bassiana;Enzyme purification;In silico;Molecular docking;Structural elucidation;β-glucosidase
Issue Date: Apr-2024
Publisher: Elsevier BV
Source: Magwaza, B. et al. 2024. Biochemical and in silico structural properties of a thermo-acid stable β-glucosidase from Beauveria bassiana. Heliyon. 10(7): 1-11. doi:10.1016/j.heliyon.2024.e28667
Journal: Heliyon; Vol. 10, Issue 7 
Abstract: 
β-glucosidase hydrolyses the glycosidic bonds in cellobiose and cello-oligosaccharides, a critical step in the saccharification for biofuel production. Hence, the aim of this study was to gain insights into the biochemical and structural properties of a β-glucosidase from Beauveria bassiana, an entomopathogenic fungus. The β-glucosidase was purified to homogeneity using salt precipitation, ultrafiltration, and chromatographic techniques, attaining a specific activity of 496 U/mg. The molecular mass of the enzyme was then estimated via SDS-PAGE to be 116 kDa, while its activity pattern was confirmed by zymography using 4-methylumbelliferyl-β-d-glucopyranoside. Furthermore, the pH optima and temperature of the enzyme were found to be pH 5.0 and 60 °C respectively; its activity was significantly enhanced by Mg2+ and Na+ and was found to be relatively moderate in the presence of ethanol and dichloromethane. Molecular docking of the modelled B. bassiana β-glucosidase structure with the substrates, viz., 4-nitrophenyl β-d-glucopyranoside and cellobiose, revealed the binding affinity energies of -7.2 and -6.2 (kcal mol-1), respectively. Furthermore, the computational study predicted Lys-657, Asp-658, and Arg-1000 as the core amino acid residues in the catalytic site of the enzyme. This is the first investigation into a purified β-glucosidase from B. bassiana, providing valuable insights into the functional properties of carbohydrases from entomopathogenic fungal endophytes.
URI: https://hdl.handle.net/10321/5747
ISSN: 2405-8440
DOI: 10.1016/j.heliyon.2024.e28667
Appears in Collections:Research Publications (Applied Sciences)

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