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Title: A cluster-randomized controlled trial to reduce Diarrheal disease and dengue entomological risk factors in rural primary schools in Colombia
Authors: Overgaard, Hans J. 
Alexander, Neal 
Matiz, María Inés 
Jaramillo, Juan Felipe 
Olano, Victor Alberto 
Vargas, Sandra Lucía 
Sarmiento, Diana 
Lenhart, Audrey 
Stenström, Thor-Axel 
Issue Date: 7-Nov-2016
Publisher: National Centre for Biotechnology Information
Source: Overgaard, H. J. et al. 2016. A cluster-randomized controlled trial to reduce Diarrheal disease and dengue entomological risk factors in rural primary schools in Colombia. PLoS Neglected Tropical Diseases. 10(11): 1-26. doi: 10.1371/journal.pntd.0005106
Journal: PLoS neglected tropical diseases (Online) 
As many neglected tropical diseases are co-endemic and have common risk factors, inte-grated control can efficiently reduce disease burden and relieve resource-strained public health budgets. Diarrheal diseases and dengue fever are major global health problems sharing common risk factors in water storage containers. Where provision of clean water is inadequate, water storage is crucial. Fecal contamination of stored water is a common source of diarrheal illness, but stored water also provides breeding sites for dengue vector mosquitoes. Integrating improved water management and educational strategies for both diseases in the school environment can potentially improve the health situation for students and the larger community. The objective of this trial was to investigate whether interven-tions targeting diarrhea and dengue risk factors would significantly reduce absence due to diarrheal disease and dengue entomological risk factors in schools.

Methodology/Principal Findings
A factorial cluster randomized controlled trial was carried out in 34 rural primary schools
(1,301 pupils) in La Mesa and Anapoima municipalities, Cundinamarca, Colombia. Schools were randomized to one of four study arms: diarrhea interventions (DIA), dengue interven-tions (DEN), combined diarrhea and dengue interventions (DIADEN), and control (CON). Interventions had no apparent effect on pupil school absence due to diarrheal disease (p = 0.45) or on adult female Aedes aegypti density (p = 0.32) (primary outcomes). However, the dengue interventions reduced the Breteau Index on average by 78% (p = 0.029), with Breteau indices of 10.8 and 6.2 in the DEN and DIADEN arms, respectively compared to 37.5 and 46.9 in the DIA and CON arms, respectively. The diarrhea interventions improved water quality as assessed by the amount of Escherichia coli colony forming units (CFU); the ratio of Williams mean E. coli CFU being 0.22, or 78% reduction (p = 0.008).

Integrated control of dengue and diarrhea has never been conducted before. This trial pres-ents an example for application of control strategies that may affect both diseases and the first study to apply such an approach in school settings. The interventions were well received and highly appreciated by students and teachers. An apparent absence of effect in primary outcome indicators could be the result of pupils being exposed to risk factors out-side the school area and mosquitoes flying in from nearby uncontrolled breeding sites. Inte-grated interventions targeting these diseases in a school context remain promising because of the reduced mosquito breeding and improved water quality, as well as educa-tional benefits. However, to improve outcomes in future integrated approaches, simulta-neous interventions in communities, in addition to schools, should be considered; using appropriate combinations of site-specific, effective, acceptable, and affordable interventions.
ISSN: 1935-2727 (print)
1935-2735 (online)
DOI: 10.1371/journal.pntd.0005106
Appears in Collections:Research Publications (Academic Support)

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