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Title: Biodiesel synthesis from microalgae using immobilized Aspergillus niger whole cell lipase biocatalyst
Authors: Guldhe, Abhishek 
Singh, Poonam 
Kumari, Sheena K. 
Rawat, Ismail 
Permaul, Kugen 
Bux, Faizal 
Keywords: Whole cell lipase;Microalgae;Scenedesmus obliquus;Biodiesel;Aspergillus niger
Issue Date: 2016
Publisher: Elsevier
Source: Guldhe, A. et al. 2016. Biodiesel synthesis from microalgae using immobilized Aspergillus niger whole cell lipase biocatalyst. Renewable Energy. 85: 1002-1010.
Journal: Renewable energy (Online) 
Whole cell lipase catalysis and microalgal feedstocks make overall biodiesel synthesis greener and sustainable. In this study, a novel approach of whole cell lipase-catalyzed conversion of Scenedesmus obliquus lipids was investigated for biodiesel synthesis. Microalgal biodiesel was characterized for its fuel properties. Optimization of process parameters for immobilized Aspergillus niger whole cell lipase-catalyzed biodiesel synthesis was carried out. Highest biodiesel conversion of 53.76% was achieved from S. obliquus lipids at 35 °C, methanol to oil ratio of 5:1 and 2.5% water content based on oil weight with 6 BSPs (Biomass support particles). Step-wise methanol addition was applied to account for methanol tolerance, which improved biodiesel conversion upto 80.97% and gave 90.82 ± 1.43% yield. Immobilized A. niger lipase can be used for 2 batches without significant loss in conversion efficiency. Most of the fuel properties of biodiesel met the specifications set by international standards.
ISSN: 0960-1481 (print)
1879-0682 (online)
Appears in Collections:Research Publications (Applied Sciences)

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