Please use this identifier to cite or link to this item: http://hdl.handle.net/10321/1562
Title: Carbon utilization profile of a thermophilic fungus, Thermomyces lanuginosus using phenotypic microarray
Authors: Mchunu, Nokuthula Peace
Permaul, Kugen 
Alam, Maqsudul
Singh, Suren 
Keywords: Thermophilic;Carbon Source;Hexose;Pentose
Issue Date: Sep-2013
Publisher: Scientific Research Publishing
Source: Nokuthula, P.N. Permaul, K. Alam, M & Singh, S. 2013. Carbon utilization profile of a thermophilic fungus, Thermomyces lanuginosus using phenotypic microarray. Advances in Bioscience and Biotechnology, 4: 24-32
Abstract: The thermophilic filamentous fungus, Thermomyces lanuginosus produces the largest amount of xylanase reported. In addition to this, it expresses large amount of other enzymes that have been used indus- trially or have academic interest. Thus, this fungus has a potential to be applied for biomass conversion to produce biofuel or other applications. In this study, the Biolog system was used to characterize the utilisa- tion and growth of T. lanuginosus on 95 carbon sources. The carbohydrates based compounds, both single sugars and oligosaccharide, showed the best utilisation profile, with the pentose sugar xylose in- ducing the highest growth, followed by trehelose, raf- finose, D-mannose turanose fructose and glucose. Among oligosaccharides, sucrose had the highest my- celium formation followed by stachyose, maltose, maltotriose, glycogen and dextrin. Interestingly the fungus also grew well on cellobiose suggesting that this fungus can produce cellulose hydrolysing pro- teins. D-alanine was the best amino acid to promote fungal growth while the effect of other amino acids tested was similar to the control. These results dem- onstrate the ability of this fungus to grow relatively well on most plant based compounds thus making this fungus a possible candidate for plant biomass conver- sion which can be applied to a number of biotechno- logical applications including biofuel production.
URI: http://hdl.handle.net/10321/1562
ISSN: 2156-8456
Appears in Collections:Research Publications (Health Sciences)

Files in This Item:
File Description SizeFormat 
mchunu__permaul__alam___singh_2013_adv_in_biosc___biotech.pdf4.67 MBAdobe PDFThumbnail
View/Open
Show full item record

Page view(s)

177
checked on Jul 22, 2018

Download(s)

84
checked on Jul 22, 2018

Google ScholarTM

Check


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.