Please use this identifier to cite or link to this item:
https://hdl.handle.net/10321/5445
DC Field | Value | Language |
---|---|---|
dc.contributor.advisor | Permaul, Kugen | - |
dc.contributor.advisor | Govender, Algasan | - |
dc.contributor.advisor | Puri, Adarsh Kumar | - |
dc.contributor.author | Naidoo, Krinolen Krishna Rajahrathanum | en_US |
dc.date.accessioned | 2024-09-02T07:28:18Z | - |
dc.date.available | 2024-09-02T07:28:18Z | - |
dc.date.issued | 2024-05 | - |
dc.identifier.uri | https://hdl.handle.net/10321/5445 | - |
dc.description | Submitted in fulfillment for the Degree of Master of Applied Science in Biotechnology, Durban University of Technology, Durban, South Africa, 2024. | en_US |
dc.description.abstract | Zygomycetes are known for their relatively high chitosan content (approximately 10% m/m) in comparison with other fungal genera. In this study, Mucor circinelloides was grown on the following industrial waste substrates: corn steep liquor (CSL); soft drink overflow spillage waste (DBW); and sugarcane molasses (MOL). Biomass production on waste substrates was statistically optimized by Plackett-Burman design in conjunction with Response Surface Methodology, followed by validation of the model. DBW hindered fungal biomass growth and was found to be a statistically insignificant variable and therefore omitted from further optimizations. The validated model produced a biomass of 77.87 g/L, a 2.65-fold increase over the highest-yielding unoptimized medium. Fungal biomass obtained after batch fermentation was subjected to acid-alkaline treatment for chitin extraction from the cell wall and deacetylation of the chitin to chitosan. A yield of 8-9% chitosan was obtained from the fungal biomass. FTIR spectroscopic analysis was conducted on the extracted fungal chitosan to compare extracted chitosan against commercial chitosan and chitosan monomer. The waste-grown, fungal-derived chitosan profiles were similar to those of commercial crustacean chitosan. The extracted chitosan was used in conjunction with additives and solvent systems to create biopolymer variants with differing properties. A library of data from the chitosan biopolymer variants was generated with considerable differences in characteristics based on their composition. Improvements in sample #11 (the most modified formulation) in contrast to the most common chitosan biopolymer film composition used in literature (sample #9), included a 3.37-fold improvement in the static force required to break the film. There was a 3.39-fold increase in tensile strength and an 11-fold reduction in elongation (%) and elongation rates. The creation of these variants will allow the use of these chitosan biopolymers for specific industrial applications. | en_US |
dc.format.extent | 116 p | en_US |
dc.language.iso | en | en_US |
dc.subject | Waste substrates | en_US |
dc.subject.lcsh | Biomass energy | en_US |
dc.subject.lcsh | Water--Waste | en_US |
dc.subject.lcsh | Biopolymers | en_US |
dc.subject.lcsh | Zygomycetes | en_US |
dc.title | Development of chitosan biopolymer films by fungal fermentation of waste substrates | en_US |
dc.type | Thesis | en_US |
dc.description.level | M | en_US |
dc.identifier.doi | https://doi.org/10.51415/10321/5445 | - |
local.sdg | SDG03 | en_US |
local.sdg | SDG06 | en_US |
item.languageiso639-1 | en | - |
item.openairetype | Thesis | - |
item.cerifentitytype | Publications | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
item.fulltext | With Fulltext | - |
item.grantfulltext | open | - |
Appears in Collections: | Theses and dissertations (Applied Sciences) |
Files in This Item:
File | Description | Size | Format | |
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Naidoo_KKR_2024.pdf | 2.85 MB | Adobe PDF | View/Open |
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