Please use this identifier to cite or link to this item: http://hdl.handle.net/10321/2990
Title: Improving the survival of probiotic in simulated conditions and azoxymethane-induced colon tumour bearing mice using modified citrus pectin-alginate microencapsulation
Authors: Odun-Ayo, Frederick Oluwasheyi 
Mellem, John Jason 
Reddy, Lalini 
Keywords: Modified citrus pectin;Alginate;Probiotic;Microencapsulation
Issue Date: 2016
Publisher: PKP Publishing Services Network
Source: Odun-Ayo, F. 2016. Improving the survival of probiotic in simulated conditions and azoxymethane-induced colon tumour bearing mice using modified citrus pectin-alginate microencapsulation. African Journal of Traditional, Complementary and Alternative Medicines. 13(2): 101-109.
Abstract: Background: For a probiotic to be viable it needs to be preserved at a recommended minimum level of 6–7 log10cfu/g in the product being consumed, as suggested by the International Dairy Federation. Different biopolymer matrices have been used for encapsulation of probiotic; however, loss of viability is still a challenge. Materials and Methods: Modified citrus pectin-alginate microbeads containing Lactobacillus acidophilus ATCC 4356 was developed. Efficiency of the microbeads was evaluated in simulated conditions of the gastrointestinal tract and in Balb/c mice induced with colon tumor. Genomic identification of faecal lactobacilli samples from treated mice was also performed. Results: The Modified citrus pectin-alginate probiotic microbeads significantly enhanced the viability of Lactobacillus acidophilus ATCC 4356 compared to the control (p< 0.05) both in vitro and in vivo. Exposure of the modified citrus pectin-alginate microbeads to 3 hours of simulated gastric juice resulted in 82.7% survival of L. acidophilus ATCC 4356. Also, the number of faecal lactobacilli in the modified citrus pectin-alginate probiotic treated mice increased by 10.2% after 28 days. Conclusion: Modified citrus pectin-alginate is a novel effective means of oral delivery of bacterial cells and bioactive compounds. Modified citrus pectin-alginate can be used in probiotic therapy which may improve the prevention of colon cancer.
URI: http://hdl.handle.net/10321/2990
ISSN: 0189-6016 (print)
2505-0044 (online)
Appears in Collections:Research Publications (Applied Sciences)

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