2026-09-19T14:58:51Zhttps://openscholar.dut.ac.za/server/oai/request

oai:openscholar.dut.ac.za:10321/47072025-04-03T01:01:17Zcom_10321_5col_10321_6

An integrated approach for biofuel and fertilizer production using microalgae grown in wastewater Musetsho, Pfano Bux, Faizal Renuka, Nirmala Guldhe, Abhishek Microalgae biomass Wastewater Microalgae--Biotechnology Poultry--Manure Biomass energy--South Africa Sewage Submitted in fulfillment of the requirements for the degree of Master of Technology: Biotechnology, Durban University of Technology, Durban, South Africa, 2022. Microalgae are recognized as potential candidates for resource recovery from wastewater and are projected for biorefinery models. Therefore, this study was undertaken to evaluate the potential of poultry litter and municipal wastewater as nutrient and water sources, for the cultivation of Acutodesmus obliquus for lipids production for biodiesel application. The efficacy of lipid extracted biomass (LEA) as fertilizer for mung bean crops was also assessed in microcosm. A. obliquus cultivation in acid pre-treated poultry litter extract (PPLE) showed maximum biomass production of 1.90 g L-1 , which was 74.67% and 12.61% higher than the raw poultry litter extract (RPPE) and BG11 respectively. Higher NO3-N, NH3-N, and PO4-P removal of 79.51%, 81.82%, and 80.52% respectively were observed in PPLE as compared to RPLE treatment. The highest biomass (140.36 mg L-1 d -1 ), lipids (38.49 mg L-1 d -1 ), and carbohydrates (49.55 mg L-1 d -1 ) productivities were observed in the PPLE medium. The application of LEA as a fertilizer for mung bean crops showed improvement in plant growth and soil microbial activity. A maximum increase in organic carbon (59.5%) and dehydrogenase activity (130.8%) was observed in LEA amended soil which was significantly higher than chemical fertilizer (CF) control in 30 days. Whilst plant fresh weight and leaf chlorophyll in the LEA amended soil was comparable to whole algal biomass (WA) and CF control. The findings of the present study could be a basis for sustainable biorefinery for the valorization of wastewater for the production of microalgae-derived biofuel and byproducts for agricultural applications. M 2023-04-13T07:26:04Z 2023-04-13T07:26:04Z 2022-09 Thesis https://hdl.handle.net/10321/4707 https://doi.org/10.51415/10321/4707 en 126 p application/pdf