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Comparative analysis of genetically modified maize by implementation of a half-seed extraction technique Pienaar, Fernando Permaul, Kugen Corn Crops Plant genetic engineering Crop improvement Biotechnology--Dissertations, Academic Corn--Genetic engineering Crops--Genetic engineering Corn--Biotechnology Submitted in fulfilment of the requirements for the Degree of Master of Technology: Biotechnology, Durban University of Technology, Durban, South Africa, 2007. The development of transgenic plants resulted in the need to utilize the various molecular methods (e.g., ELISA, real - time PCR etc.) for the detection or analysis of the presence or absence of a specific trait in a particular plant (Bt in this study). The overall aim of this study was to optimize a half – seed extraction technique as part of a laboratory protocol for transgenic maize plants and to explore the possibility of using the following molecular techniques: horizontal isoelectric focusing, real - time PCR and ELISA, as methods for detection of the Bt trait for incorporation into the half – seed extraction protocol. M 2008-07-16T12:46:25Z 2008-07-16T12:46:25Z 2007 Thesis 308421 http://hdl.handle.net/10321/307 https://doi.org/10.51415/10321/307 en 81 p application/pdf