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Title: Comparative analysis of genetically modified maize by implementation of a half-seed extraction technique
Authors: Pienaar, Fernando 
Keywords: Corn;Crops;Plant genetic engineering;Crop improvement;Biotechnology--Dissertations, Academic
Issue Date: 2007
The development of transgenic plants resulted in the need to utilize the various molecular methods (e.g., ELISA, real - time PCR etc.) for the detection or analysis of the presence or absence of a specific trait in a particular plant (Bt in this study). The overall aim of this study was to optimize a half – seed extraction technique as part of a laboratory protocol for transgenic maize plants and to explore the possibility of using the following molecular techniques: horizontal isoelectric focusing, real - time PCR and ELISA, as methods for detection of the Bt trait for incorporation into the half – seed extraction protocol.
Submitted in fulfilment of the requirements for the Degree of Master of Technology: Biotechnology, Durban University of Technology, Durban, South Africa, 2007.
Appears in Collections:Theses and dissertations (Applied Sciences)

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