Please use this identifier to cite or link to this item: http://hdl.handle.net/10321/307
Title: Comparative analysis of genetically modified maize by implementation of a half-seed extraction technique
Authors: Pienaar, Fernando
Keywords: Corn--Genetic engineering;Crops--Genetic engineering;Corn--Biotechnology;Plant genetic engineering;Crop improvement;Biotechnology--Dissertations, Academic
Issue Date: 2007
Abstract: The development of transgenic plants resulted in the need to utilize the various molecular methods (e.g., ELISA, real - time PCR etc.) for the detection or analysis of the presence or absence of a specific trait in a particular plant (Bt in this study). The overall aim of this study was to optimize a half – seed extraction technique as part of a laboratory protocol for transgenic maize plants and to explore the possibility of using the following molecular techniques: horizontal isoelectric focusing, real - time PCR and ELISA, as methods for detection of the Bt trait for incorporation into the half – seed extraction protocol.
Description: Thesis (M.Tech.: Biotechnology)-Dept. of Biotechnology and Food Technology, Durban University of Technology, 2007 iv, 75 leaves
URI: http://hdl.handle.net/10321/307
Appears in Collections:Theses and dissertations (Applied Sciences)

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