Please use this identifier to cite or link to this item: http://hdl.handle.net/10321/847
DC FieldValueLanguage
dc.contributor.authorSingh, Suren-
dc.date.accessioned2013-04-08T07:17:25Z-
dc.date.available2013-04-08T07:17:25Z-
dc.date.issued2012-06-08-
dc.identifier.citationLi Zhou, Dan-Dan Niu, Kang-Ming Tian, Xian-Zhong Chen, Bernard A. Prior, Wei Shen, Gui-Yang Shi, Suren Singh, Zheng-Xiang Wang. 'Genetically switched d-lactate production in Escherichia coli.' Metabolic Engineering. 14,5 (2012): 560-568. 10.1016/j.ymben.2012.05.004.en_US
dc.identifier.urihttp://hdl.handle.net/10321/847-
dc.description.abstractDuring a fermentation process, the formation of the desired product during the cell growth phase competes with the biomass for substrates or inhibits cell growth directly, which results in a decrease in production efficiency. A genetic switch is required to precisely separate growth from production and to simplify the fermentation process. The ldhA promoter, which encodes the fermentative d-lactate dehydrogenase (LDH) in the lactate producer Escherichia coli CICIM B0013-070 (ack-pta pps pflB dld poxB adhE frdA), was replaced with the λ pR and pL promoters (as a genetic switch) using genomic recombination and the thermo-controllable strain B0013–070B (B0013-070, ldhAp::kan-cIts857-pR–pL), which could produce two-fold higher LDH activity at 42 °C than the B0013-070 strain, was created. When the genetic switch was turned off at 33 °C, strain B0013-070B produced 10% more biomass aerobically than strain B0013-070 and produced only trace levels of lactate which could reduce the growth inhibition caused by oxygen insufficiency in large scale fermentation. However, 42 °C is the most efficient temperature for switching on lactate production. The volumetric productivity of B0013-070B improved by 9% compared to that of strain B0013-070 when it was grown aerobically at 33 °C with a short thermo-induction at 42 °C and then switched to the production phase at 42 °C. In a bioreactor experiment using scaled-up conditions that were optimized in a shake flask experiment, strain B0013-070B produced 122.8 g/l d-lactate with an increased oxygen-limited productivity of 0.89 g/g·h. The results revealed the effectiveness of using a genetic switch to regulate cell growth and the production of a metabolic compound.en_US
dc.format.extent9en_US
dc.language.isoenen_US
dc.publisherElsevieren_US
dc.subjectGenetic switchen_US
dc.subjectThermo-regulationen_US
dc.subjectd-Lactate productionen_US
dc.subjectLambda pL and pR tandem promotersen_US
dc.subjectLdhAen_US
dc.subject.lcshEscherichia colien_US
dc.subject.lcshLactate dehydrogenaseen_US
dc.titleGenetically switched D-lactate production in Escherichia colien_US
dc.typeArticleen_US
dc.publisher.urihttp://www.sciencedirect.com/science/article/pii/S1096717612000651en_US
dc.dut-rims.pubnumDUT-001786
item.languageiso639-1other-
item.fulltextNo Fulltext-
item.grantfulltextnone-
Appears in Collections:Research Publications (Applied Sciences)
Show simple item record

Page view(s) 50

632
checked on Feb 19, 2020

Google ScholarTM

Check


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.